Wheat superoxide dismutase isoenzymes

D. S. Robinson, J. K. Donnelly, S. M. Lawlor, P. J. Frazier, N. W R Daniels

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7 Citations (Scopus)


Evidence was obtained indicating that the superoxide dismutase (SOD) A and B isoenzymes of the wheat cultivar Tonic are of the Cu/ Zn type, whereas the SOD-C group are similar to manganese containing dismutases. Heat inactivation plots showed that the enzymic activity in crude extracts was relatively stable up to 50°C. The thermodynamic parameters enthalpy, ΔH#, free energy, ΔG#, and entropy, ΔS#, were estimated for the thermal inactivation of extracted SOD activity and a purified SOD-C isoenzyme. The SOD-C isoenzyme was purified extensively and shown to contain two closely related tetrametic isozymes (pI6·0 and 66·1) of Mr 80 000. A Mr of 20 000 has been calculated for the subunits. The N-terminal amino acid sequence of the purified SOD-C could be aligned with that of the Mn-SOD enzyme of maize and showed 57% homology.

Original languageEnglish
Pages (from-to)93-101
Number of pages9
JournalJournal of Cereal Science
Issue number1
Publication statusPublished - Jan 1996


  • Cereals
  • Heat inactivation
  • Superoxide dismutase
  • Wheat


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