Visualisation of CGRP and ChAT-like immunoreactivity in identified trigeminal neurones by combined peroxidase and alkaline phosphatase enzymatic reactions

T. F C Batten, K. Appenteng, S. Saha

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

We report here a method that allows simultaneous visualisation of two antigens within single neurones. In essence this involves the combined use of horseradish peroxidase and alkaline phosphatasereactions to visualise two markers. Using this method we show that ChAT-and CGRP-like immunoreactivity can be co-localised within single neurones of the V to VII motor nuclei. In the case of the V motor nucleus, we show that each marker can be localised in motoneurones labelled with horseradish peroxidase.
Original languageEnglish
Pages (from-to)314-324
Number of pages11
JournalBrain Research
Volume447
Issue number2
DOIs
Publication statusPublished - 3 May 1988
Externally publishedYes

Keywords

  • Alkaline phosphatase
  • Calcitonin gene-related peptide
  • Choline acetyltransferase
  • Horseradish peroxidase
  • Immunohistochemistry
  • Trigeminal neuron

Fingerprint

Dive into the research topics of 'Visualisation of CGRP and ChAT-like immunoreactivity in identified trigeminal neurones by combined peroxidase and alkaline phosphatase enzymatic reactions'. Together they form a unique fingerprint.

Cite this