Use of protein A-coated colloidal gold particles for immunoelectronmicroscopic localization of ACTH on ultrathin sections

Research output: Contribution to journalArticlepeer-review

Original languageEnglish
Pages (from-to)317-20
Number of pages4
JournalHistochemistry
Volume60
Issue number3
DOIs
Publication statusPublished - Jan 1979

ACTH was localized in dissociated porcine adenohypophysial cells using a novel indirect EM immunocytochemical technique. Incubation of ultrathin resin sections in anti-ACTH was followed by incubation with protein A-coated colloidal gold particles. Protein A binds specifically to the Fc part of the IgG molecule, and thus the ACTH-containing secretory granules became labelled with electron-dense gold particles. With this method, the dissociated porcine ACTH cells was identified as containing numerous round or ovoid 170--300 nm secretory granules.

    Research areas

  • Adrenocorticotropic Hormone, Animals, Antigen-Antibody Reactions, Cells, Cultured, Colloids, Cytoplasmic Granules, Gold, Microscopy, Electron, Pituitary Gland, Anterior, Staphylococcal Protein A, Swine

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