Use of protein A-coated colloidal gold particles for immunoelectronmicroscopic localization of ACTH on ultrathin sections

T F Batten; C R Hopkins

doi:10.1007/BF00500659

Use of protein A-coated colloidal gold particles for immunoelectronmicroscopic localization of ACTH on ultrathin sections

T F Batten, C R Hopkins

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

ACTH was localized in dissociated porcine adenohypophysial cells using a novel indirect EM immunocytochemical technique. Incubation of ultrathin resin sections in anti-ACTH was followed by incubation with protein A-coated colloidal gold particles. Protein A binds specifically to the Fc part of the IgG molecule, and thus the ACTH-containing secretory granules became labelled with electron-dense gold particles. With this method, the dissociated porcine ACTH cells was identified as containing numerous round or ovoid 170--300 nm secretory granules.

Original language	English
Pages (from-to)	317-20
Number of pages	4
Journal	Histochemistry
Volume	60
Issue number	3
DOIs	https://doi.org/10.1007/BF00500659
Publication status	Published - Jan 1979

Keywords

Adrenocorticotropic Hormone
Animals
Antigen-Antibody Reactions
Cells, Cultured
Colloids
Cytoplasmic Granules
Gold
Microscopy, Electron
Pituitary Gland, Anterior
Staphylococcal Protein A
Swine

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10.1007/BF00500659

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title = "Use of protein A-coated colloidal gold particles for immunoelectronmicroscopic localization of ACTH on ultrathin sections",

abstract = "ACTH was localized in dissociated porcine adenohypophysial cells using a novel indirect EM immunocytochemical technique. Incubation of ultrathin resin sections in anti-ACTH was followed by incubation with protein A-coated colloidal gold particles. Protein A binds specifically to the Fc part of the IgG molecule, and thus the ACTH-containing secretory granules became labelled with electron-dense gold particles. With this method, the dissociated porcine ACTH cells was identified as containing numerous round or ovoid 170--300 nm secretory granules.",

keywords = "Adrenocorticotropic Hormone, Animals, Antigen-Antibody Reactions, Cells, Cultured, Colloids, Cytoplasmic Granules, Gold, Microscopy, Electron, Pituitary Gland, Anterior, Staphylococcal Protein A, Swine",

author = "Batten, \{T F\} and Hopkins, \{C R\}",

year = "1979",

month = jan,

doi = "10.1007/BF00500659",

language = "English",

volume = "60",

pages = "317--20",

journal = "Histochemistry",

issn = "0301-5564",

publisher = "Springer Nature",

number = "3",

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T1 - Use of protein A-coated colloidal gold particles for immunoelectronmicroscopic localization of ACTH on ultrathin sections

AU - Batten, T F

AU - Hopkins, C R

PY - 1979/1

Y1 - 1979/1

N2 - ACTH was localized in dissociated porcine adenohypophysial cells using a novel indirect EM immunocytochemical technique. Incubation of ultrathin resin sections in anti-ACTH was followed by incubation with protein A-coated colloidal gold particles. Protein A binds specifically to the Fc part of the IgG molecule, and thus the ACTH-containing secretory granules became labelled with electron-dense gold particles. With this method, the dissociated porcine ACTH cells was identified as containing numerous round or ovoid 170--300 nm secretory granules.

AB - ACTH was localized in dissociated porcine adenohypophysial cells using a novel indirect EM immunocytochemical technique. Incubation of ultrathin resin sections in anti-ACTH was followed by incubation with protein A-coated colloidal gold particles. Protein A binds specifically to the Fc part of the IgG molecule, and thus the ACTH-containing secretory granules became labelled with electron-dense gold particles. With this method, the dissociated porcine ACTH cells was identified as containing numerous round or ovoid 170--300 nm secretory granules.

KW - Adrenocorticotropic Hormone

KW - Animals

KW - Antigen-Antibody Reactions

KW - Cells, Cultured

KW - Colloids

KW - Cytoplasmic Granules

KW - Gold

KW - Microscopy, Electron

KW - Pituitary Gland, Anterior

KW - Staphylococcal Protein A

KW - Swine

U2 - 10.1007/BF00500659

DO - 10.1007/BF00500659

M3 - Article

C2 - 224008

SN - 0301-5564

VL - 60

SP - 317

EP - 320

JO - Histochemistry

JF - Histochemistry

IS - 3

ER -

Use of protein A-coated colloidal gold particles for immunoelectronmicroscopic localization of ACTH on ultrathin sections

Abstract

Keywords

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