Use of protein A-coated colloidal gold particles for immunoelectronmicroscopic localization of ACTH on ultrathin sections

T F Batten, C R Hopkins

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

ACTH was localized in dissociated porcine adenohypophysial cells using a novel indirect EM immunocytochemical technique. Incubation of ultrathin resin sections in anti-ACTH was followed by incubation with protein A-coated colloidal gold particles. Protein A binds specifically to the Fc part of the IgG molecule, and thus the ACTH-containing secretory granules became labelled with electron-dense gold particles. With this method, the dissociated porcine ACTH cells was identified as containing numerous round or ovoid 170--300 nm secretory granules.

Original languageEnglish
Pages (from-to)317-20
Number of pages4
JournalHistochemistry
Volume60
Issue number3
DOIs
Publication statusPublished - Jan 1979

Keywords

  • Adrenocorticotropic Hormone
  • Animals
  • Antigen-Antibody Reactions
  • Cells, Cultured
  • Colloids
  • Cytoplasmic Granules
  • Gold
  • Microscopy, Electron
  • Pituitary Gland, Anterior
  • Staphylococcal Protein A
  • Swine

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