Abstract
ACTH was localized in dissociated porcine adenohypophysial cells using a novel indirect EM immunocytochemical technique. Incubation of ultrathin resin sections in anti-ACTH was followed by incubation with protein A-coated colloidal gold particles. Protein A binds specifically to the Fc part of the IgG molecule, and thus the ACTH-containing secretory granules became labelled with electron-dense gold particles. With this method, the dissociated porcine ACTH cells was identified as containing numerous round or ovoid 170--300 nm secretory granules.
Original language | English |
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Pages (from-to) | 317-20 |
Number of pages | 4 |
Journal | Histochemistry |
Volume | 60 |
Issue number | 3 |
DOIs | |
Publication status | Published - Jan 1979 |
Keywords
- Adrenocorticotropic Hormone
- Animals
- Antigen-Antibody Reactions
- Cells, Cultured
- Colloids
- Cytoplasmic Granules
- Gold
- Microscopy, Electron
- Pituitary Gland, Anterior
- Staphylococcal Protein A
- Swine