Use of protein A-coated colloidal gold particles for immunoelectronmicroscopic localization of ACTH on ultrathin sections

T F Batten, C R Hopkins

    Research output: Contribution to journalArticlepeer-review

    31 Citations (Scopus)

    Abstract

    ACTH was localized in dissociated porcine adenohypophysial cells using a novel indirect EM immunocytochemical technique. Incubation of ultrathin resin sections in anti-ACTH was followed by incubation with protein A-coated colloidal gold particles. Protein A binds specifically to the Fc part of the IgG molecule, and thus the ACTH-containing secretory granules became labelled with electron-dense gold particles. With this method, the dissociated porcine ACTH cells was identified as containing numerous round or ovoid 170--300 nm secretory granules.

    Original languageEnglish
    Pages (from-to)317-20
    Number of pages4
    JournalHistochemistry
    Volume60
    Issue number3
    DOIs
    Publication statusPublished - Jan 1979

    Keywords

    • Adrenocorticotropic Hormone
    • Animals
    • Antigen-Antibody Reactions
    • Cells, Cultured
    • Colloids
    • Cytoplasmic Granules
    • Gold
    • Microscopy, Electron
    • Pituitary Gland, Anterior
    • Staphylococcal Protein A
    • Swine

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