The effect of centrally injected CDP-choline on respiratory system: Involvement of phospholipase to thromboxane signaling pathway

Bora B. Topuz, Burcin Altinbas, Mustafa S. Yilmaz, Sikha Saha, Trevor F. Batten, Vahide Savci, Murat Yalcin

Research output: Contribution to journalArticlepeer-review

Abstract

CDP-choline is an endogenous metabolite in phosphatidylcholine biosynthesis. Exogenous administration of CDP-choline has been shown to affect brain metabolism and to exhibit cardiovascular, neuroendocrine neuroprotective actions. On the other hand, little is known regarding its respiratory actions and/or central mechanism of its respiratory effect. Therefore the current study was designed to investigate the possible effects of centrally injected CDP-choline on respiratory system and the mediation of the central cholinergic receptors and phospholipase to thromboxane signaling pathway on CDP-choline-induced respiratory effects in anaesthetized rats.Intracerebroventricularly (i.c.v.) administration of CDP-choline induced dose- and time-dependent increased respiratory rates, tidal volume and minute ventilation of male anaesthetized Spraque Dawley rats. İ.c.v. pretreatment with atropine failed to alter the hyperventilation responses to CDP-choline whereas mecamylamine, cholinergic nicotinic receptor antagonist, mepacrine, phospholipase A2 inhibitor, and neomycin phospholipase C inhibitor, blocked completely the hyperventilation induced by CDP-choline. In addition, central pretreatment with furegrelate, thromboxane A2 synthesis inhibitor, also partially blocked CDP-choline-evoked hyperventilation effects.These data show that centrally administered CDP-choline induces hyperventilation which is mediated by activation of central nicotinic receptors and phospholipase to thromboxane signaling pathway.
Original languageEnglish
Pages (from-to)50-58
Number of pages9
JournalRespiratory Physiology and Neurobiology
Volume195
Early online date18 Feb 2014
DOIs
Publication statusPublished - 1 May 2014
Externally publishedYes

Keywords

  • CDP-choline
  • Central cholinergic receptors
  • Central prostaglandinergic system
  • Intracerebroventricular
  • Minute ventilation
  • Respiratory frequency
  • Tidal volume

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