TY - JOUR
T1 - The effect of centrally injected CDP-choline on respiratory system
T2 - Involvement of phospholipase to thromboxane signaling pathway
AU - Topuz, Bora B.
AU - Altinbas, Burcin
AU - Yilmaz, Mustafa S.
AU - Saha, Sikha
AU - Batten, Trevor F.
AU - Savci, Vahide
AU - Yalcin, Murat
PY - 2014/5/1
Y1 - 2014/5/1
N2 - CDP-choline is an endogenous metabolite in phosphatidylcholine biosynthesis. Exogenous administration of CDP-choline has been shown to affect brain metabolism and to exhibit cardiovascular, neuroendocrine neuroprotective actions. On the other hand, little is known regarding its respiratory actions and/or central mechanism of its respiratory effect. Therefore the current study was designed to investigate the possible effects of centrally injected CDP-choline on respiratory system and the mediation of the central cholinergic receptors and phospholipase to thromboxane signaling pathway on CDP-choline-induced respiratory effects in anaesthetized rats.Intracerebroventricularly (i.c.v.) administration of CDP-choline induced dose- and time-dependent increased respiratory rates, tidal volume and minute ventilation of male anaesthetized Spraque Dawley rats. İ.c.v. pretreatment with atropine failed to alter the hyperventilation responses to CDP-choline whereas mecamylamine, cholinergic nicotinic receptor antagonist, mepacrine, phospholipase A2 inhibitor, and neomycin phospholipase C inhibitor, blocked completely the hyperventilation induced by CDP-choline. In addition, central pretreatment with furegrelate, thromboxane A2 synthesis inhibitor, also partially blocked CDP-choline-evoked hyperventilation effects.These data show that centrally administered CDP-choline induces hyperventilation which is mediated by activation of central nicotinic receptors and phospholipase to thromboxane signaling pathway.
AB - CDP-choline is an endogenous metabolite in phosphatidylcholine biosynthesis. Exogenous administration of CDP-choline has been shown to affect brain metabolism and to exhibit cardiovascular, neuroendocrine neuroprotective actions. On the other hand, little is known regarding its respiratory actions and/or central mechanism of its respiratory effect. Therefore the current study was designed to investigate the possible effects of centrally injected CDP-choline on respiratory system and the mediation of the central cholinergic receptors and phospholipase to thromboxane signaling pathway on CDP-choline-induced respiratory effects in anaesthetized rats.Intracerebroventricularly (i.c.v.) administration of CDP-choline induced dose- and time-dependent increased respiratory rates, tidal volume and minute ventilation of male anaesthetized Spraque Dawley rats. İ.c.v. pretreatment with atropine failed to alter the hyperventilation responses to CDP-choline whereas mecamylamine, cholinergic nicotinic receptor antagonist, mepacrine, phospholipase A2 inhibitor, and neomycin phospholipase C inhibitor, blocked completely the hyperventilation induced by CDP-choline. In addition, central pretreatment with furegrelate, thromboxane A2 synthesis inhibitor, also partially blocked CDP-choline-evoked hyperventilation effects.These data show that centrally administered CDP-choline induces hyperventilation which is mediated by activation of central nicotinic receptors and phospholipase to thromboxane signaling pathway.
KW - CDP-choline
KW - Central cholinergic receptors
KW - Central prostaglandinergic system
KW - Intracerebroventricular
KW - Minute ventilation
KW - Respiratory frequency
KW - Tidal volume
UR - http://www.scopus.com/inward/record.url?scp=84896051234&partnerID=8YFLogxK
U2 - 10.1016/j.resp.2014.02.005
DO - 10.1016/j.resp.2014.02.005
M3 - Article
C2 - 24560778
AN - SCOPUS:84896051234
SN - 1569-9048
VL - 195
SP - 50
EP - 58
JO - Respiratory Physiology and Neurobiology
JF - Respiratory Physiology and Neurobiology
ER -