Increased GABAB receptor subtype expression in the nucleus of the solitary tract of the spontaneously hypertensive rat

Emma J. Spary, Azhar Maqbool, Sikha Saha, Trevor F C Batten

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Expression of GABAB receptor messenger RNA (mRNA) in the central nervous system was compared between the spontaneously hypertensive (SHR) and normotensive Wistar Kyoto (WKY) rat. Polymerase chain reaction (PCR) revealed all the isoforms except B1e in cortex, hypothalamus, and medulla oblongata. In the nucleus of the solitary tract (NTS) and ventrolateral medulla (VLM), the B1a-c and 1 g isoforms were present as well as B2. Real-time PCR detected significantly higher levels of B1a (p < 0.01) and B2 (p < 0.05) mRNA in the NTS of SHR compared to WKY. A significant increase in B1a expression (p < 0.05) was detected in VLM. Immunolabeling suggested presynaptic and postsynaptic expression of B1a, B1b, and B2 subtypes throughout the NTS, with significant differences in distribution patterns and labeling between subtypes and between SHR and WKY. These findings suggest that GABAB receptors expressed by neurones in NTS may be involved in cardiovascular regulation and that changes in GABAB mRNA expression levels may contribute to the hypertensive state in SHR.
Original languageEnglish
Pages (from-to)211-224
Number of pages14
JournalJournal of Molecular Neuroscience
Volume35
Issue number2
DOIs
Publication statusPublished - Jun 2008
Externally publishedYes

Keywords

  • GABA receptor
  • Hypothalamus
  • Immunohistochemistry
  • Nucleus tractus solitarius
  • Polymerase chain reaction
  • Splice variant
  • Ventrolateral medulla

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