Effects of TRH and somatostatin on releases of prolactin and growth hormone in vitro by the pituitary of Poecilia latipinna: II. Electron-microscopic morphometry using automatic image analysis

T F Batten, T Wigham

Research output: Contribution to journalArticlepeer-review

Abstract

Pituitary glands from a teleost fish were incubated in the presence of the synthetic hypophysiotropic peptides, thyrotrophin-releasing hormone and somatostatin, in two media of different osmotic pressure. The effects on prolactin and growth hormone cells were detected by electron-microscopic morphometry with the aid of an image analyser. Thyrotrophin-releasing hormone caused changes in prolactin cell ultrastructure consistent with stimulated hormone release and, in the low osmotic pressure medium, appeared to increase synthetic activity. There was no effect on growth hormone cells. After somatostatin treatment, both synthesis and release in prolactin cells appeared to be inhibited, and there was an obvious inhibition of synthesis and release in growth hormone cells. The response of both cell types to somatostatin did not appear to be dependent on the osmotic pressure of the medium.
Original languageEnglish
Pages (from-to)595-603
Number of pages9
JournalCell and Tissue Research
Volume237
Issue number3
DOIs
Publication statusPublished - Aug 1984
Externally publishedYes

Keywords

  • Animals
  • Female
  • Fishes
  • Growth Hormone
  • In Vitro Techniques
  • Microscopy, Electron
  • Pituitary Gland
  • Prolactin
  • Somatostatin
  • Subcellular Fractions
  • Thyrotropin-Releasing Hormone

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