Changes in glutamate transporter expression in mouse forebrain areas following focal ischemia

Research output: Contribution to journalArticlepeer-review

  • Pirusha Ketheeswaranathan
  • Neil A. Turner
  • Emma J. Spary
  • Trevor F C Batten
  • Barry W. McColl
  • Sikha Saha
Original languageEnglish
Pages (from-to)93-103
Number of pages11
JournalBrain Research
Volume1418
Early online date19 Aug 2011
DOIs
Publication statusPublished - 18 Oct 2011
Externally publishedYes
Dysfunction of glutamate transporters has been proposed to promote neuronal death in modelled cerebral ischemia. However, these studies have produced conflicting results and the changes in glutamate transporter expression have not yet been examined in a mouse focal ischemic stroke model. This study used quantitative real-time reverse-transcription polymerase chain reaction to examine glutamate transporter mRNA expression in the hippocampus, cortex and striatum in a mouse model of focal ischemic stroke induced by middle cerebral artery occlusion (MCAO). Effects on mRNA expression of glial (GLT-1, GLAST) and neuronal (EAAC1) glutamate transporters in these brain areas were assessed by comparing MCAO brains with sham-operated control brains. Changes in transporter proteins were also assessed by immunohistochemistry using specific antibodies to GLT-1 and GLAST. Following focal ischemia, GLT-1 mRNA expression was decreased significantly in the ipsilateral hippocampus and cortex compared to the sham-operated brains (p < 0.05). There were no significant differences in GLAST or EAAC1 mRNA expression between MCAO and sham-operated brains. Immunohistochemistry also confirmed a marked reduction in GLT-1 immunoreactivity in the cortex and hippocampus. Down regulation of GLT-1 in these brain areas may impair normal clearance of synaptically-released glutamate and contribute to neural damage following focal ischemic insult.

    Research areas

  • GLT-1, Glutamate transporter, Middle cerebral artery occlusion, Mouse, Quantitative real time PCR, Stroke

External organisations

  • University of Leeds
  • University of Edinburgh

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